Catalog Products » Recombinant Proteins » Chemokines » Hemofiltrate CC Chemokine-1 (72aa) (HCC-1)/CCL14, Human

HCC-1/CCL14 (72aa), Human

Name: HCC-1/CCL14 (72aa), Human
SKU: Z03214-1
Rating: 5 (7 reviews)

Overview

Synonyms	NCC-2, SCYA14
Description	HCC-1/CCL14 is a member of the chemokine family, which are small chemotactic proteins that regulate cell migration under inflammatory and steady state conditions. HCC-1 is expressed in epithelial and decidual cells and is unique among chemokines due to its high abundance in normal human plasma. HCC-1 can bind to chemokine receptors CCR1 and CCR5, however full length HCC-1 is a weak agonist of CCR1 and only becomes potent after removal of its eight N-terminal residues. Chemokine decoy receptor D6 can bind HCC-1 and promote its degradation as a means to regulate its level in vivo. Functionally HCC-1 promotes trophoblast migration by regulating extracellular matrix components as well as specific adhesion molecules. Recombinant human Hemofiltrate CC Chemokine-1 (72 a.a.) (HCC-1)/CCL14 (rhHCC-1) produced in E. coli is a single non-glycosylated polypeptide chain containing 72 amino acids. A fully biologically active molecule, rhHCC-1 has a molecular mass of 8.4kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No	Q16627
Species	Human
Source	E. coli
Biological Activity	ED₅₀ < 25 µg/mL, measured by the FLIPR assay using CHO cells transfected with human CCR5, the receptor of human CCL14, corresponding to a specific activity of > 40 units/mg.
Sequence	TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN

Measured Molecular Weight	8.4 kDa, observed by reducing SDS-PAGE.
Purity	> 95% by SDS-PAGE analysis.
Formulation	Lyophilized after extensive dialysis against PBS.
Reconstitution	Reconstituted in ddH₂O at 100 μg/mL.
Endotoxin Level	< 0.2 EU/μg, determined by LAL method.
Storage	Lyophilized recombinant human Hemofiltrate CC Chemokine-1 (72 a.a.) (HCC-1)/CCL14 (rhHCC-1) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhHCC-1 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note	For research use only