ENA-78/CXCL5, Human(HEK 293-expressed)
| Species | Human | |
| Protein Construction |
|
|
| Purity | > 98% as analyzed by SDS-PAGE | |
| Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method | |
| Biological Activity | The EC50 value of human ENA-78/CXCL5 on Ca2+ mobilization assay in CHO-K1/ Gα15/hCXCR2 cells (human Gα15 and human CXCR2 stably expressed in CHO-K1 cells) is less than 200.0 ng/ml. | |
| Expression System | HEK 293 | |
| Apparent Molecular Weight | ~8.5 kDa, on SDS-PAGE under reducing conditions. | |
| Formulation | Lyophilized after extensive dialysis against PBS. | |
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. | |
| Storage & Stability | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background | Epithelial cellderived neutrophilactivating peptide (ENA78) also known as C-X-C motif chemokine 5 (CXCL5), is a small cytokine belonging to the CXC chemokine family. It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon, IFN-γ. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. Full length CXCL5 (78 aa) is trimmed at the Nterminal end by cathepsin G and chymotrypsin to ENA-74 (74 aa) and ENA-70 (70aa), with the shortened forms showing increased potency relative to full length CXCL5. CXCL5can signal through the CXCR2 receptor. |
| Synonyms | C-X-C motif chemokine 5; ENA-78; Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; Small-inducible cytokine B5; ENA78; SCYB5 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
Z02820-20 |
ENA-78/CXCL5 (5-78aa), Human - 20 μg |
$165.00 |
|
Z03261-1 |
ENA-78/CXCL5 (5-78aa), Human - 1 mg |
$1600.00 |
|
Z03271-5 |
ENA78/CXCL5(9-78 a.a.), Human - 5 μg |
$45.00 |
For more documents, please visit "Technical Support".
| Cat. No. Z03255 | ||
|---|---|---|
| Size | Price | |
| 5 μg | $45.00 | |
| 25 μg | $100.00 | |
