|Description||Interleukin-22 (IL-22) is a member of a group of cytokines called the IL-10 family which include IL-10,IL-19, IL-20, IL-24, and IL-26. IL-22 shares use of the IL-10R2 in cell signaling with other members of this family IL-22 signals through a receptor system consisting of IL-10R-ß/CRF2-4 and IL-22R, both of which are members of the class II cytokine-receptor family. IL-22 is produced by activated DCs and T cells and initiates an innate immune response against bacterial pathogens especially in epithelial cells such as those in the respiratory tract and gut. IL-22 along with IL-17 is rapidly produced by splenic LTi-like cells and can also be produced by Th17 cells, which plays a likely role in the coordinated response of both adaptive and innate immune systems.
Recombinant MouseIL-22 produced in E. coli is a single non-glycosylated polypeptide chain containing 147 amino acids. A fully biologically active molecule, rmIL-22 has a molecular mass of 16.8 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||ED50 < 0.5 ng/ml. Measured by its ability to induce IL-10 secretion in COLO 205 (human colon carcinoma cells).|
Leu34-Val179 (Accession #: Q9JJY9), expressed with an N-terminal Met
|Measured Molecular Weight||16.8 kDa, observed by reducing SDS-PAGE|
|Purity||> 98% as analyzed by SDS-PAGE& HPLC.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Mouse IL-22 remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Mouse IL-22 should be stable up to 1 week at 4°C or up to 3 months at -20°C.|
|Note||For research use only|
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