|Synonyms||Vascular Endothelial Growth Factor, VPF,Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen|
|Description||Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.
Recombinant Mouse Vascular Endothelial Growth Factor A164 (VEGF-A164) produced in E.coli is a single, non-glycosylated polypeptide chain containing 165 amino acids.A fully biologically active molecule, recombinant Mouse VEGF-A164 has a molecular mass of 19.4 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||The ED50 < 5 ng/mL, measured by a cell proliferation assay using HUVEC Cells, corresponding to a specific activity of > 2× 105 units/mg.|
MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP
DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH
IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK
NTDSRCKARQ LELNERTCRC DKPRR
|Measured Molecular Weight||19.4kDa, observed by reducing SDS-PAGE.|
|Purity||> 98% as analyzed by SDS-PAGE.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||<0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Mouse Vascular Endothelial Growth Factor A164 (VEGF-A164) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Mouse VEGF-A164 should be stable up to 1 week at 4°C or up to 3 months at -20°C.For long term storage it is recommended that a carrier protein (example 0.1% BSA) can be added. Avoid repeated freeze-thaw cycles.|
|Note||For research use only|
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