|Synonyms||SP-D; SFTPD; PSP-D; COLEC7; PSPD; SFTP4|
|Description||Surfactant protein D is a member of the collectin family of innate immune modulators. It is constitutively secreted by alveolar lining cells and epithelium associated with tubular structures, that contains a N-terminal collagen-like domain and a C-terminal lectin domain that are characteristic of members of the collectin family of proteins, and also shows calcium-dependent binding to specific saccharides. Besides it is involved in the development of acute and chronic inflammation of the lung. Several human lung diseases are characterized by decreased levels of bronchoalveolar SP-D.
Recombinant Human SP-D produced in HEK 293 cells is a polypeptide chain containing 355 amino acids. A fully biologically active molecule, rhSP-D has a molecular mass of 40-45 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
AEMKTYSHRT MPSACTLVMC SSVESGLPGR DGRDGREGPR
GEKGDPGLPG AAGQAGMPGQ AGPVGPKGDN GSVGEPGPKG
DTGPSGPPGP PGVPGPAGRE GPLGKQGNIG PQGKPGPKGE
AGPKGEVGAP GMQGSAGARG LAGPKGERGV PGERGVPGNT
GAAGSAGAMG PQGSPGARGP PGLKGDKGIP GDKGAKGESG
LPDVASLRQQ VEALQGQVQH LQAAFSQYKK VELFPNGQSV
GEKIFKTAGF VKPFTEAQLL CTQAGGQLAS PRSAAENAAL
QQLVVAKNEA AFLSMTDSKT EGKFTYPTGE
SLVYSNWAPG EPNDDGGSED CVEIFTNGKW NDRACGEKRL
|Measured Molecular Weight||40-45kDa, observed by reducing SDS-PAGE.|
|Purity||> 98% as analyzed by reducing SDS-PAGE.|
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||<0.2 EU/μg, determined by LAL|
|Storage||Lyophilized recombinant Human SP-D remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human SP-D should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.|
|Note||For research use only|
For more documents, please visit "Technical Support".