|Synonyms||Tumor Necrosis Factor-β, human; TNF-beta, human,TNFSF1, Lymphotoxin-alpha (LT-α)|
|Description||TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF. TNF-β is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands, and signals through TNFR1 and TNFR2. TNF-β is produced by activated T and B lymphocytes, and has similar activities to TNF-α. It mediates a large variety of inflammatory, immunostimulatory, and antiviral responses.
Recombinant Human TNF-β produced in E. coli is is a glycosylated polypeptide chain of 172 amino acids. A fully biologically active molecule,rhTNF-β has a molecular mass of 18.6 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||ED50 < 4 pg/mL, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of >2.5 x 108 units/mg.|
MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK
QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV
VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK
MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL
|Measured Molecular Weight||18.6kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Storage||Lyophilized recombinant Human Tumor Necrosis Factor-beta (TNF-β) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human TNF-β should be stable up to 1 week at 4°C or up to 3 months at -20°C.For long term storage it is recommended that a carrier protein (example 0.1% BSA) can be added. Avoid repeated freeze-thaw cycles.|
|Note||For research use only|
For more documents, please visit "Technical Support".