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Catalog Products » Recombinant Proteins » Enterokinase (EK), His, Lyophilized, Bovine

Enterokinase (EK), His, Lyophilized, Bovine

Synonyms Enteropeptidase, ENTK, PRSS7
Description Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys(DDDDK↑). Enterokinase will not work if the recognition site is followed by a proline. rbEKhas the highest activity than EK of other species and is used wildly in biochemical applications. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.
Recombinant Bovine Enterokinase (His-tagged) (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids, 6 × His at C-terminus. A fully biologically active molecule, rbEK has a molecular mass of 40 kDa and is obtained by proprietary chromatographic techniques at GenScript.
Species Bovine
Source P. pastoris
Biological Activity 100 IU/μg

Measured Molecular Weight 40 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE.
Formulation Lyophilized from a 0.2 μm filtered solution in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2.
Reconstitution Reconstitute lyophilized EK powder in sterile deionized water at 1mg/ml, then dilute in EK Dilution/Storage Buffer according to your requirement.
Endotoxin Level <0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Bovine Enterokinase (His-tagged) (rbEK) remains stable up to one year at -20°C from date of receipt. Please avoid freeze-thaw cycles.
Note For research use only

Cat. No.
Product Name
Enterokinase, His, Bovine - 100 IU

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Cat. No. Z03376
10 ug $60.00
50 ug $90.00
1 mg $675.00