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EGF Fc Chimera, Human

Name: Protein
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Overview

Description	Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through the EGF receptor (EGFR) also known as erbB1, is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). EGF-receptor binding results in cellular proliferation, differentiation, and survival. EGF is a low-molecular-weight polypeptide first purified from the mouse submandibular gland, but since then found in many human tissues including submandibular gland, parotid gland. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Recombinant Human EGF Fc Chimera produced in CHO cells is a polypeptide chain containing 286 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhEGF has a molecular mass of 33-37 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Source	CHO
Biological Activity	ED₅₀ < 1 ng/ml, measured in a cell proliferation assay using Balb/3T3 cells.
Sequence	NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELRIEGRMDD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK

Properties

Measured Molecular Weight	33-37 kDa, observed by reducing SDS-PAGE.
Purity	> 95% as analyzed by reducing SDS-PAGE.
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstituted in ddH₂O or PBS at 100 μg/ml.
Endotoxin Level	< 0.2 EU/μg, determined by LAL method.
Storage	Lyophilized recombinant Human EGF Fc Chimera remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human EGF should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.