|Description||Noggin, also known as NOG, is a homodimeric glycoprotein that bindsto and modulates the activity of TGF-beta family ligands. It is expressed in condensing cartilage and immature chondrocytes. Noggin antagonizes bone morphogenetic protein (BMP) activities by blocking epitopes on BMPs needed for binding to their receptors. Noggin has been shown to be involved in many developmental processes, such as neural tube formation and joint formation. During development, Noggin diffuses through extracellular matrices and forms morphogenic gradients, regulating cellular responses dependent on the local concentration of the signaling molecule.
Recombinant Human Noggin Fc Chimera produced in CHO cells is a polypeptide chain containing 438 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhNoggin has a molecular mass of 57 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||ED50<60ng/ml, measured in a bioassay using ATDC5 cells in the presence of 10ng/ml human BMP-4.|
QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR
SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL
LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM
WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP
EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE
CKCSCIEGRM DDKTHTCPPC PAPELLGGPS VFLFPPKPKD
TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT
KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP
APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV
KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK
LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK
|Measured Molecular Weight||57 kDa, observed by reducing SDS-PAGE.|
|Purity||> 97% as analyzed by reducing SDS-PAGE.|
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Human Noggin remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human Noggin should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.|
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