×
Catalog Products » Recombinant Proteins » CRISPR/Cas9-related Protein » GenCrispr Cas9 Nuclease

GenCrispr Cas9 Nuclease



Description GenCrispr Cas9 Nuclease is the recombinant Streptococcus pyogenes Cas9 (wt) protein purified from E. coli and can be used for genome editing by inducing site-specific double stranded breaks in double stranded DNA. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. The RNP complex recognizes the target site by matching gRNA with the genomic DNA sequence and leads to DNA breaks within 3 bases from the NGG PAM (Protospacer Adjacent Motif). With GenCrispr Cas9 nuclease, customers can screen highly efficient gRNA using in vitro DNA cleavage assays. It could be a powerful tool for gene editing.

Product Source : GenCrispr Cas9 Nuclease is produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes without nuclear localization signal (NLS).

With this Cas9 Nuclease, you can:
  1. Screening for highly efficient and specific targeting gRNAs by in vitro DNA cleavage using Cas9 Nuclease from S. pyogenes ( please find the detailed protocol in the product manual ).
  2. Highly purified Cas9 antigen could be used for specific antibody production.

Storage GenCrispr Cas9 is supplied with 1x storage buffer (10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol PH7.4 at 25℃) and is recommended to be stored at -20℃.

GenCrispr Cas9 Nuclease
GenCrispr Cas9 Nuclease

In vitro DNA cleavage assay with Cas9 nuclease
Reactions were set up according to recommended conditions, and cleavage products were resolved on a 1% agarose gel. Input DNA is EcoRV-linearized pUC57 plasmid DNA




Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".
Cat. No. Z03386
Size
Price
50 μg (5×10μg)(0.2mg/ml) $105.00
10 μg (0.2mg/ml) $33.00
24 hour
 
*
*
*
*