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In vitro DNA cleavage assay with GenCrispr NLS-Cas9-EGFP nuclease
Reactions were set up according to recommended conditions, and cleavage products were resolved on a 1% agarose gel. Input DNA is EcoR V-linearized pUC57 plasmid DNA. Lane 1, DNA + gRNA; lane 2, marker; lane 3 and 4, DNA + gRNA + NLS-Cas9-EGFP 100 ng (lane 3) or 50 ng (lane 4).
Left: Cell transfection assay. 12 h after electroporation, cells were observed under bright or fluorescence microscope.
Right: in vivo gene editing efficiency assay by T7E1. Lane 1, marker; lane 2, negative control; lane 3, gRNA + NLS-Cas9-EGFP; lane 4, gRNA + NLS-Cas9.
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