Catalog Products » GenCRISPR™ Cas9 Genome Editing Products » GenCRISPR™ Nucleases » GenCrispr T7 Endonuclease I
GenCrispr T7 Endonuclease I

The T7E1 assay to detect the cleavage efficiency of control HPRT gRNA with Cas9 protein.

GenCrispr T7 Endonuclease I

T7 Endonuclease I (T7E1) recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, hetero duplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5´ to the mismatch. The protein is the product of T7 gene 3. GenCrispr T7 Endonuclease I is a fusion protein produced from E.coli.
Z03396
$55.00

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Description

T7 Endonuclease I (T7E1) recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, hetero duplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5´ to the mismatch. The protein is the product of T7 gene 3. GenCrispr T7 Endonuclease I is a fusion protein produced from E.coli.

Note

T7 Endonuclease I is a structure-selective enzyme. It acts on a variety of DNA substrates with different specific activities. It is important to control the amount of enzyme and the reaction time used for cleavage of a particular substrate. Temperatures above 42°C cause an increase in nonspecific nuclease activity and should be avoided.

Applications
  • Resolve four-way junction or branched DNA.
  • Detect or cleave hetero duplex and nicked DNA.
  • Randomly cleave linear DNA for shot-gun cloning.
  • Detect gene mutagenesis and SNPs, for cleavage efficiency assays induced by TALEN,CRISPR/CAS9 or other gene editing tools.

Properties
Storage & Stability GenCrispr T7 Endonuclease I is supplied in 1X storage buffer (200 mM NaCl,20 mM Tris-HCl(pH 7.5), 0.1 mM EDTA, 1 mM dithiothreitol, 0.15% Triton X-100 and 50% glycerol). The recommended storage temperature is -20°C.

Examples
  • GenCrispr T7 Endonuclease I
  • GenCrispr T7 Endonuclease I

    The T7E1 assay to detect the cleavage efficiency of control HPRT gRNA with Cas9 protein.


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