T7 Endonuclease I (T7E1) recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, hetero duplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5´ to the mismatch. The protein is the product of T7 gene 3. GenCrispr T7 Endonuclease I is a fusion protein produced from E.coli.
Resolve four-way junction or branched DNA
Detect or cleave hetero duplex and nicked DNA
Randomly cleave linear DNA for shot-gun cloning
Detect gene mutagenesis and SNPs, for cleavage efficiency assays induced by TALEN,CRISPR/CAS9 or other gene editing tools.
GenCrispr T7 Endonuclease I is supplied in 1X storage buffer (200 mM NaCl,20 mM Tris-HCl（pH 7.5）, 0.1 mM EDTA, 1 mM dithiothreitol, 0.15% Triton X-100 and 50% glycerol). The recommended storage temperature is -20°C.