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Home » Gene Synthesis & Molecular Biology Services » B7-H2/ICOSLG His, Human

B7-H2/ICOSLG His, Human

Synonyms ICOSLG; B7-H2; B7H2; B7RP-1; B7RP1; CD275; GL50; ICOS-L; ICOSL; LICOS; ICOS ligand
Description B7-H2, best known as the ligand of inducible costimulator, belongs to B7-CD28 family, is a transmembrane glycoprotein of approximately 60 kDa. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, dendritic cells, and also in monocytes, and is a ligand for CD28 and CTLA-4 in human, whereas these interactions are not conserved in mouse. B7-H2 and B7-1 or B7-2 interacted with CD28 through distinctive domains. B7-H2-CD28 interaction is essential for the costimulation of human T cells' primary responses to allogeneic antigens and memory recall responses.
Recombinant Human B7-H2/ICOSLG produced in HEK293 cells is a polypeptide chain containing 250 amino acids with C-terminal 10His. A fully biologically active molecule, rhB7-H2/ICOSLG has a molecular mass of 45-55 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No O75144
Species Human
Source HEK293
Biological Activity Immobilized B7-H2-6His, Human at 1μg/mL (100 μL/well) can bind ICOS Fc Chimera, Human(Z03412) with a linear range of 2.29-185.2ng/mL.
Sequence
DTQEKEVR AMVGSDVE LSCACPEG SRFDLNDV YVYWQTSE
SKTVVTYH IPQNSSLE NVDSRYRN RALMSPAG MLRGDFSL
RLFNVTPQ DEQKFHCL VLSQSLGF QEVLSVEV TLHVAANF
SVPVVSAP HSPSQDEL TFTCTSIN GYPRPNVY WINKTDNS
LLDQALQN DTVFLNMR GLYDVVSV LRIARTPS VNIGCCIE
NVLLQQNL TVGSQTGN DIGERDKI TENPVSTG EKNAATWS
HHHHHHHH HH

Measured Molecular Weight 45-55 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by reducing SDS-PAGE.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol.
Reconstitution Reconstituted in ddH₂O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant B7-H2/ICOSLG, His, Human remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human B7-H2/ICOSLG should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
Note For research use only

  • B7-H2/ICOSLG His, Human
  • B7-H2/ICOSLG His, Human

    Immobilized B7-H2-6His,Human at 1μg/mL (100 μL/well) can bind ICOS Fc Chimera, Human(Z03412) with a linear range of 2.29-185.2ng/mL.


Cat. No.
Product Name
Price
Z03409-50
B7-1(CD80) Fc Chimera, Human - 50 ug
$159.00
Z03416-1
B7-2/CD86 Fc Chimera, Human - 1 mg
$1200.00
Z03452-1
B7-2/CD86 Fc His, Human - 1 mg
$1200.00
Z03414-1
B7-H2/ICOSLG Fc Chimera, Human - 1 mg
$1200.00
Z03426-100
B7-H3 Fc Chimera, Human - 100 ug
$275.00
Z03427-100
B7-H3, His, Human - 100 ug
$395.00
 


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Cat. No. Z03415
Size
Price
100 μg $250.00
1 mg $1200.00