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Home » Gene Synthesis & Molecular Biology Services » B7-2/CD86 Fc Chimera, Human

B7-2/CD86 Fc Chimera, Human

Synonyms Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2
Description B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs), both are type 1 transmembrane proteins with a membrane distal IgV and a membrane proximal IgC domain. They share ~25% sequence homology and interact with the same receptors, CD28 and CTLA-4.Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. T cell activation requires engagement of the T cell receptor (TCR) with the peptide–MHC complex presented on the cell surface of antigen presenting cells (APCs). In addition to this antigen-specific interaction, a second interaction involving costimulatory receptors (CD28, ICOS) on T cells and their respective ligands (B7-1/B7-2, ICOS-L) on APCs is required for optimal T cell activation. B7-1 and B7-2 may also function to deliver signal into dendritic cells. While B7-1 favors binding to CTLA-4, B7-2 shows a preference for CD28.
Recombinant Human B7-2 Fc Chimera produced in HEK293 cells is a polypeptide chain containing 461 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhB7-2 has a molecular mass of 65-80 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P42081
Species Human
Source HEK293
Biological Activity Immobilized B7-2CD86/-Fc at 5 μg/mL (100 μL/well) can bind human Biotin-CD28-Fc with a linear range of 0.09~3.12μg/mL when detected by Streptavidin-HRP.
Sequence
LSGAAPLK IQAYFNET ADLPCQFA NSQNQSLS ELVVFWQD
QENLVLNE VYLGKEKF DSVHSKYM GRTSFDSD SWTLRLHN
LQIKDKGL YQCIIHHK KPTGMIRI HQMNSELS VLANFSQP
EIVPISNI TENVYINL TCSSIHGY PEPKKMSV LLRTKNST
IEYDGVMQ KSQDNVTE LYDVSISL SVSFPDVT SNMTIFCI
LETDKTRL LSSPFSIE LEDPQPPP DHIP

Measured Molecular Weight 65-80 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by reducing SDS-PAGE.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant B7-2 Fc Chimera, Human remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human B7-2 Fc Chimera should be stable up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
Note For research use only

  • B7-2/CD86 Fc Chimera, Human
  • B7-2/CD86 Fc Chimera, Human

    Immobilized B7-2/CD86-Fc at 5 μg/mL (100 μL/well) can bind human Biotin-CD28-Fc with a linear range of 0.09~3.12μg/mL.


Cat. No.
Product Name
Price
Z03409-50
B7-1(CD80) Fc Chimera, Human - 50 ug
$159.00
Z03452-1
B7-2/CD86 Fc His, Human - 1 mg
$1200.00
Z03414-1
B7-H2/ICOSLG Fc Chimera, Human - 1 mg
$1200.00
Z03415-1
B7-H2/ICOSLG His, Human - 1 mg
$1200.00
Z03426-100
B7-H3 Fc Chimera, Human - 100 ug
$275.00
Z03427-100
B7-H3, His, Human - 100 ug
$395.00
 


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Cat. No. Z03416
Size
Price
100 μg $250.00
1 mg $1200.00