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Home » Gene Synthesis & Molecular Biology Services » PVR/CD155 Fc Chimera, Human

PVR/CD155 Fc Chimera, Human

Synonyms CD155 antigen; CD155; HVED; NECL5; Necl-5; nectin-like 5; Nectin-like protein 5; poliovirus receptor; PVR
Description PVR is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates. PVR's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. PVR/CD155 was originally isolated based on its ability to mediate polio virus attachment to host cells. The full length (or PVR alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20aa signal sequence, a 323aa extracellular region, a 24aa TM segment and a 50aa cytoplasmic tail. PVR binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homotypically. PVR is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin.
Recombinant Human PVR/CD155 Fc Chimera produced in HEK293 cells is a polypeptide chain containing 555 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhPVR/CD155 has a molecular mass of 85-105 kDa, analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P15151
Species Human
Source HEK293
Biological Activity Immobilized PVR/CD155 Fc, human (Z03435) at 5 μg/mL (100 μL/well) can bind Biotin-TIGIT Fc, Human (Z03439) with a linear range of 6.1-48.8 ng/mL when detected by Streptavidin-HRP.
Sequence
WPPPGTGD VVVQAPTQ VPGFLGDS VTLPCYLQ VPNMEVTH
VSQLTWAR HGESGSMA VFHQTQGP SYSESKRL EFVAARLG
AELRNASL RMFGLRVE DEGNYTCL FVTFPQGS RSVDIWLR
VLAKPQNT AEVQKVQL TGEPVPMA RCVSTGGR PPAQITWH
SDLGGMPN TSQVPGFL SGTVTVTS LWILVPSS QVDGKNVT
CKVEHESF EKPQLLTV NLTVYYPP EVSISGYD NNWYLGQN
EATLTCDA RSNPEPTG YNWSTTMG PLPPFAVA QGAQLLIR
PVDKPINT TLICNVTN ALGARQAE LTVQVKEG PPSEHSGI
SRN

Measured Molecular Weight 85-105 kDa, observed by reducing SDS-PAGE.
Purity > 97% as analyzed by reducing SDS-PAGE.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant PVR/CD155 Fc Chimera, Human remains stable for up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human PVR/CD155 Fc Chimera should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
Note For research use only

  • PVR/CD155 Fc Chimera, Human
  • PVR/CD155 Fc Chimera, Human

    Immobilized PVR/CD155 Fc, human at 5 μg/mL (100 μL/well) can bind Biotin-TIGIT Fc, Human with a linear range of 6.1-48.8 ng/mL.


Cat. No.
Product Name
Price
Z03436-1
PVR/CD155 His, Human - 1 mg
$2200.00
 



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Cat. No. Z03435
Size
Price
100 μg $250.00
1 mg $1200.00