Immobilized ACE-2 Fc Chimera, Human (Cat. No. Z03484) at 2 μg/mL can bind SARS-CoV-2 Spike protein (RBD, His & Avi Tag, CHO-expressed) (Cat. No. Z03512) with a serial dilution.
THETM His Tag Antibody [HRP], mAb, Mouse (Cat.No.A00612) is used as a secondary antibody (0.2 μg/mL).
Lane 1: 2μg of SARS-CoV-2 Spike protein (RBD, His & Avi Tag, CHO-expressed), reducing(R)
Lane 2: 2μg of SARS-CoV-2 Spike protein (RBD, His & Avi Tag, CHO-expressed), non-reducing(NR)
> 95% as analyzed by SDS-PAGE
Species | SARS-CoV-2 | ||||||
Protein Construction |
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Purity | > 95% as analyzed by SDS-PAGE | ||||||
Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method | ||||||
Biological Activity | SARS-CoV-2 Spike protein (RBD, Avi & His Tag, CHO-expressed) can bind with Human ACE2 (Cat. No.: Z03484) in functional ELISA assay. | ||||||
Expression System | CHO | ||||||
Theoretical Molecular Weight | 30 kDa | ||||||
Formulation | Supplied as a solution in PBS pH 7.4 | ||||||
Concentration | Please refer to the COA for the specific lot. | ||||||
Storage & Stability | Upon receiving, this product remains stable for up to 6 months at -20°C or below. Avoid repeated freeze-thaw cycles. |
Immobilized ACE-2 Fc Chimera, Human (Cat. No. Z03484) at 2 μg/mL can bind SARS-CoV-2 Spike protein (RBD, His & Avi Tag, CHO-expressed) (Cat. No. Z03512) with a serial dilution.
THETM His Tag Antibody [HRP], mAb, Mouse (Cat.No.A00612) is used as a secondary antibody (0.2 μg/mL).
Lane 1: 2μg of SARS-CoV-2 Spike protein (RBD, His & Avi Tag, CHO-expressed), reducing(R)
Lane 2: 2μg of SARS-CoV-2 Spike protein (RBD, His & Avi Tag, CHO-expressed), non-reducing(NR)
> 95% as analyzed by SDS-PAGE
Target Background | SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. It is believed that SARS-CoV-2 Spike Protein (RBD) has potential value for the diagnosis of the virus. |
Synonyms | SARS-CoV-2 SP RBD; 2019-nCoV SP RBD |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
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