Catalog Products » SARS-CoV-2 Spike protein (RBD, N501Y, Avi & His Tag)
SARS-CoV-2 Spike Protein (RBD, N501Y, His & Avi Tag)

Immobilized ACE-2 Fc Chimera, Human (Cat. No. Z03484) at 2 μg/mL can bind SARS-CoV-2 Spike protein (RBD, N501Y, His & Avi Tag) (Cat. No. Z03533) with a serial dilution.
THETM His Tag Antibody [HRP], mAb, Mouse (Cat.No.A00612) is used as a secondary antibody (0.2 μg/mL).

SARS-CoV-2 Spike Protein (RBD, N501Y, His & Avi Tag)

Loaded ACE-2 Fc Chimera, Human (CHO-expressed) Protein (Cat. No. Z03516) on Protein A Biosensor, can bind SARS-CoV-2 Spike protein (RBD, N501Y, His & Avi Tag) (Cat. No. Z03533) with an affinity constant of 2.11 nM as determined in BLI assay.

SARS-CoV-2 Spike Protein (RBD, N501Y, His & Avi Tag)

Lane 1: 1μg of SARS-CoV-2 Spike protein (RBD, N501Y, His & Avi Tag), reducing(R)
> 95% as analyzed by SDS-PAGE

SARS-CoV-2 Spike protein (RBD, N501Y, His & Avi Tag)

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein mutation N501Y is one of six key contact residues within the receptor-binding domain (RBD) and has been identified as increasing binding affinity to human and murine ACE2.
Z03533
$380.00

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Product Introduction
Species SARS-CoV-2
Protein Construction
Spike RBD [Arg319-Ser591 (N501Y)]
Accession # P0DTC2
Avi Poly-His
N-term C-term
Purity > 90% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity SARS-CoV-2 Spike protein (RBD, N501Y, Avi & His Tag) can bind with human ACE2 (Cat. No.: Z03484) in functional ELISA assay.
Expression System 293 Cells
Theoretical Molecular Weight 30 kDa
Formulation Supplied as a solution in PBS, pH 7.4.
Concentration Please refer to the COA for the specific lot.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at -20°C or below. Avoid repeated freeze-thaw cycles.

Examples
  • SARS-CoV-2 Spike Protein (RBD, N501Y, His & Avi Tag)
  • SARS-CoV-2 Spike Protein (RBD, N501Y, His & Avi Tag)

    Immobilized ACE-2 Fc Chimera, Human (Cat. No. Z03484) at 2 μg/mL can bind SARS-CoV-2 Spike protein (RBD, N501Y, His & Avi Tag) (Cat. No. Z03533) with a serial dilution.
    THETM His Tag Antibody [HRP], mAb, Mouse (Cat.No.A00612) is used as a secondary antibody (0.2 μg/mL).

  • SARS-CoV-2 Spike Protein (RBD, N501Y, His & Avi Tag)
  • SARS-CoV-2 Spike Protein (RBD, N501Y, His & Avi Tag)

    Loaded ACE-2 Fc Chimera, Human (CHO-expressed) Protein (Cat. No. Z03516) on Protein A Biosensor, can bind SARS-CoV-2 Spike protein (RBD, N501Y, His & Avi Tag) (Cat. No. Z03533) with an affinity constant of 2.11 nM as determined in BLI assay.

  • SARS-CoV-2 Spike Protein (RBD, N501Y, His & Avi Tag)
  • SARS-CoV-2 Spike Protein (RBD, N501Y, His & Avi Tag)

    Lane 1: 1μg of SARS-CoV-2 Spike protein (RBD, N501Y, His & Avi Tag), reducing(R)
    > 95% as analyzed by SDS-PAGE


Background
Target Background SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein mutation N501Y is one of six key contact residues within the receptor-binding domain (RBD) and has been identified as increasing binding affinity to human and murine ACE2.
Synonyms SARS-CoV-2 SP RBD, N501Y; 2019-nCoV SP RBD, N501Y

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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