Catalog Products » SARS-CoV-2 Spike protein (RBD, Y453F, Avi & His Tag)

SARS-CoV-2 Spike protein (RBD, Y453F, His & Avi Tag)

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Immobilized ACE-2 Fc Chimera, Human (Cat. No. Z03484) at 2 μg/mL can bind SARS-CoV-2 Spike protein (RBD, Y453F, His & Avi Tag) (Cat. No. Z03534) with a serial dilution.
THE^TM His Tag Antibody [HRP], mAb, Mouse (Cat.No.A00612) is used as a secondary antibody (0.2 μg/mL).

Lane 1: 1μg of SARS-CoV-2 Spike protein (RBD, Y453F, His & Avi Tag), reducing(R)
> 95% as analyzed by SDS-PAGE

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Product Introduction

Species

SARS-CoV-2

Protein Construction

Spike RBD [Arg319-Ser591 (Y453F)] Accession # P0DTC2	Avi	Poly-His
N-term		C-term

Purity

> 90% as analyzed by SDS-PAGE

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

SARS-CoV-2 Spike protein (RBD, Y453F, Avi & His Tag) can bind with human ACE2 (Cat. No.: Z03484) in functional ELISA assay.

Expression System

293 Cells

Theoretical Molecular Weight

30 kDa

Formulation

Supplied as a solution in PBS, pH 7.4.

Concentration

Please refer to the COA for the specific lot.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at -20°C or below. Avoid repeated freeze-thaw cycles.

Examples

Lane 1: 1μg of SARS-CoV-2 Spike protein (RBD, Y453F, His & Avi Tag), reducing(R)
> 95% as analyzed by SDS-PAGE

Background

Target Background	SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. The spike protein mutation Y453F may help the virus escape the host's immune response.
Synonyms	SARS-CoV-2 SP RBD, Y453F; 2019-nCoV SP RBD, Y453F

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.