SARS-CoV-2 Nucleocapsid Protein (D63G, R203M, D377Y), His Tag

SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2), also known as 2019-nCoV, is a positive-sense single-stranded RNA virus. It caused coronavirus disease 2019 (COVID-19). Nucleocapsid Protein is the most abundant structural protein of the coronavirus which is associated with the nucleic acid. The sublineage B.1.617.2 has been redesignated as a "variant of concern" (VOC-21APR-02) in May 2021, which spreads more quickly than the original version of the virus.

Price	$380.00
Cat. No.	Z03732-100
Size	100 μg 1 mg Buy in bulk
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Product Introduction Background Bottom Notes

Product Introduction

Species

SARS-CoV-2

Protein Construction

Expressed with the mutations of D63G, R203M, D377Y.

N protein (Met 1 - Ala 419) Accession # P0DTC9	Poly-His
N-term	C-term

Purity

> 90% as analyzed by SDS-PAGE

Biological Activity

SARS-CoV-2 Nucleocapsid Protein (D63G, R203M, D377Y), His Tag can bind with SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse (Cat. No. A02135) in functional ELISA assay.

Expression System

E.coli

Theoretical Molecular Weight

46.4 kDa

Apparent Molecular Weight

~47 kDa, on SDS-PAGE under reducing conditions.

Formulation

Supplied as a solution of 50 mM Tris-HCl, 150 mM NaCl, pH 8.0, containing 300 mM Imidazole

Concentration

Please refer to the COA for the specific lot

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Please avoid repeated freeze-thaw cycles.

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SARS-CoV-2 Nucleocapsid Protein (D63G, R203M, D377Y), His Tag

Lane R: 2 μg of SARS-CoV-2 Nucleocapsid Protein (D63G, R203M, D377Y), His Tag, reducing(R)
> 90% as analyzed by SDS-PAGE »

ELISA binding of SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse (GenScript, A02135) with SARS-CoV-2 Nucleocapsid Protein (D63G, R203M, D377Y), His Tag (GenScript, Z03732)

Coating antigen:
SARS-CoV-2 Nucleocapsid Protein (D63G, R203M, D377Y), His Tag (GenScript, Z03732), 1 μg/mL

SARS-CoV-2 Nucleocapsid Antibody (N338), mAb, mouse (GenScript, A02135) dilution start from 2 μg/ml »

Background

Target Background	SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2), also known as 2019-nCoV, is a positive-sense single-stranded RNA virus. It caused coronavirus disease 2019 (COVID-19). Nucleocapsid Protein is the most abundant structural protein of the coronavirus which is associated with the nucleic acid. The sublineage B.1.617.2 has been redesignated as a "variant of concern" (VOC-21APR-02) in May 2021, which spreads more quickly than the original version of the virus.
Synonyms	Lineage B.1.617.2; VUI-21APR-02; variant Delta
References	1. Tracking SARS-CoV-2 variants. 2. What You Need to Know About the Delta Variant.

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.