Quantitative PCR (qPCR), also known as real-time PCR, is a common technique utilized in basic research and clinical molecular diagnostic applications. It is widely used to diagnose infectious diseases, cancer, genetic-based diseases and to facilitate precision medicine.
GenScript provides customized qPCR probes and oligo primers with application-based QC standards to support your qPCR experiments.
All probes and oligo primers are manufactured in a clean room with strict control of the external environment to prevent exogenous contamination. Presence of exogenous contamination can cause an interference peak in the NTC, thus skewing qPCR results.
GenScript has optimized the synthesis and purification processes to create qPCR probes and oligo primers to provide high QC standards of qPCR materials, including high purity, optimal dye stability and fluorescent signal. Our high QC standards ensure specific and improved amplification of your template.
GenScript developed an online tool to help you design primers and probes for your quantitative PCR experiments using TaqMan. You can customize the potential PCR amplicon's size range, melting temperature (Tm) for the primers and probes, and the organism being investigated. In addition, you can decide how many primer/probe sets you want the tool to create. Target sequences can be inputted manually, however, we recommend using the GenBank Accession to input your target sequence.
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For quote, please download the qPCR probes and oligo primers template and send the form to the email address: firstname.lastname@example.org. Our technical account managers will contact you in 24 hours.