qPCR Probes and Oligo Primers

Strictly control exogenous contamination in clean room to avoid false positive signal

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Overview

Quantitative PCR (qPCR), also known as real-time PCR, is a common technique utilized in basic research and clinical molecular diagnostic applications. It is widely used to diagnose infectious diseases, cancer, genetic-based diseases and to facilitate precision medicine.

GenScript provides customized qPCR probes and oligo primers with application-based QC standards to support your qPCR experiments and MDx kit.

Competitive Advantages

Low exogenous contamination

Clean 1-2 time every day, internal monitoring of human/E.coli/COVID gene in clean room biweekly
Avoid NTC interference for credible results

High precision & sensitivity

High precision with CV value as low as 0.57%
High sensitivity with high detection rate up to 100%

Enhanced fluorescence

Optimize pH control & buffer and add extra process to increase stability of modification
Consistent Ct in different bathes ensure stability of qPCR kit

Stable quality & consistency

Consistent Ct in different bathes ensure stability of qPCR kit
Regularly fluorescence enzyme digestion increase to ensure the stability of modification between batches

Oligo Synthesis

Cases

Case 1. Exogenous contamination is ND within 45 cycles for both sample and environment in clean room
Case2. Successful detection of positive samples in multiplex PCR testing without NTC interference

A: Successful detection of positive samples in multiplex PCR testing

B: No peaks at NTC within 40 cycles in 96-well plate
Case3. Less influence on the molecular weight, purity in -20 ~ 4℃
Case4. Consistent Ct value for 3 bathes, raw materials with stable quality ensure stability of qPCR kit

Service

Type	Purification Methods*	Length (nt)	Deliverables	Lead Time and Price
qPCR probes	HPLC+	15-30	• Single tube or 96-well plate • Dry powder or solution • COA file	Please inquire
qPCR oligo	HPLC+	15-59		Please inquire

* HPLC + purification: The purification process developed and optimized for molecular diagnostics is completed in a clean laboratory. Before purification of each oligo, the column is thoroughly cleared of residues by multiple special rinsing procedures, and the different oligo are kept reasonably spaced apart and purified separately to avoid exogenous contamination to the greatest extent, with no peaks at NTC within 40 cycles. For more information on other purification methods, please ask for details.

Please find more customized QC information in Oligo Quality Control Service>>.

GenScript provides ISO 13485 certified qPCR probes and oligo for customers who need clinical declaration of kits.

Modification/Labeling/ Multiple Labeling: click & view the modification list>>