Custom CRISPR Plasmid Ordering

Search our CRISPR sgRNA Database to find pre-validated sgRNA sequences from the human and mouse genome.

Click here to order Broad Institute Plasmids that you can modify yourself.

Select custom designed CRISPR Plasmids below.

#1 Plasmid Ordering Information:

1. Cas9 System:

eSpCas9  New! SpCas9   SpCas9 Nickase   SaCas9   SAM (Transcription Activation)  

2. Delivery System:

Plasmid   Lentiviral   AAV   ALL  

3. Vector Type:

All-in-one Vector   Dual/Multi Vector   ALL  

4. Vector Selection
  Vector Cas9 System Delivery System Vector Type Selection Markers Cas9 Promoter Size Price
eSpCas9-2A- GFP (PX458) eSpCas9 Plasmid All-in-one Vector AmpR
EGFP
CBh 9.3 kb $199
eSpCas9-2A- Puro (PX459) V2.0 eSpCas9 Plasmid All-in-one Vector AmpR
PuroR
CBh 9.3 kb $199
eSpCas9-LentiCRISPR v2 eSpCas9 Lentiviral All-in-one Vector AmpR
PuroR
CBh 9.3 kb $199
pSpCas9 BB-2A-GFP (PX458) SpCas9 Plasmid All-in-one Vector AmpR
EGFP
CBh 9.3 kb $199
pSpCas9 BB-2A-Puro (PX459) v2.0 SpCas9 Plasmid All-in-one Vector AmpR
PuroR
CBh 9.3 kb $199
pLentiCRISPR v2 SpCas9 Lentiviral All-in-one Vector AmpR
PuroR
EFS 9.3 kb $199
pLentiGuide-Puro SpCas9
SpCas9 Nickase
Lentiviral Dual Vector gRNA AmpR
PuroR
9.3 kb $199
pGS-gRNA SpCas9 Plasmid Dual Vector- gRNA AmpR 2.7 kb $199
pGS-gRNA-Neo SpCas9 Plasmid Dual Vector- gRNA AmpR
NeoR
4.9 kb $199
pAAV_SpGuide acceptor (PX552) SpCas9 AAV Dual Vector- gRNA AmpR
EGFP
5.3 kb $199
pSpCas9n-BB (PX460) SpCas9 Nickase Plasmid All-in-one Vector AmpR CBh 8.5 kb $199
pSpCas9n-BB-2A-GFP (PX461) SpCas9 Nickase Plasmid All-in-one Vector AmpR
EGFP
CBh 9.3 kb $199
pSPCas9n-BB-2A-Puro (PX462) v2.0 SpCas9 Nickase Plasmid All-in-one Vector AmpR
PuroR
CBh 9.2 kb $199
pX601_AAV SaCas9 AAV All-in-one Vector AmpR CMV 7.4 kb $199
sgRNA (MS2) SAM Plasmid Multi Vector- gRNA AmpR 3.0 kb $199
pLenti_sgRNA(MS2)_zeo SAM Lentiviral Multi Vector- gRNA AmpR
ZeoR
BeloR
10.0 kb $199

5. Co-Vector Selection
  Vector Cas9 System Delivery System Vector Type Selection Markers Cas9 Promoter Size Price
pLentiCas9-Blast SpCas9 Lentiviral Dual Vector- Cas9 AmpR
BsdR
BleoR
EFS 12.8 kb Free with pLentiGuide-Puro
pLentiCas9-EGFP SpCas9 Lentiviral Dual Vector- Cas9 AmpR
EGFP
EFS 13.2 kb Free with pLentiGuide-Puro
pSpCas9 (PX165) SpCas9 Plasmid Dual Vector- Cas9 AmpR CBh 8.2 kb Free with pSpCas9 PX165
pAAV-SpCas9 (PX551) SpCas9 AAV Dual Vector- Cas9 AmpR pMecp2 7.4 kb Free with pAAV-SpCas9 PX551
plentiCas9n(D10A)-Blast SpCas9 Nickase Lentiviral Dual Vector- Cas9n AmpR
BsdR
BleoR
EFS 12.9 kb Free with pLentiGuide-Puro
pLenti_dCas9-VP64_Blast SAM Lentiviral Multi Vector- dCas9 AmpR
BlastR
BleoR
EF1a 14.1 kb $50 with pLenti_sgRNA(MS2)_zeo pSgRNA(MS2)
pLenti_dCas9-VP64_GFP SAM Lentiviral Multi Vector- dCas9 AmpR
EGFP
BleoR
EF1a 14.4 kb $50 with pLenti_sgRNA(MS2)_zeo pSgRNA(MS2)
pLenti_MS2-P65-HSF1_Hygro SAM Lentiviral Multi Vector- Activator AmpR
HygroR
BleoR
11.7 kb $50 with pLenti_sgRNA(MS2)_zeo pSgRNA(MS2)
pLenti_MS2-P65-HSF1_GFP SAM Lentiviral Multi Vector- Activator AmpR
EGFP
BleoR
11.4 kb $50 with pLenti_sgRNA(MS2)_zeo pSgRNA(MS2)

6. gRNA Sequence Selection
Synthesize my specified gRNA sequence
Design and synthesize gRNA sequences for my target sequence
*Plasmid name:
*Species:
*
*Plasmid name:
*Species:



7. Plasmid delivery
Research Grade 4μg Free
HT Transfection Grade 10μg +$29
HT Transfection Grade 30μg +$49
Industrial Grade 100μg +$99
Industrial Grade 1mg +$259

Service Requests

Need additional help? Share your experiment details with our PhD-level technical support staff. Click the “Submit to Technical Support” option below.

We recommend using 2 or more unique gRNA for each gene you wish to target, in order to increase the likelihood of successful genome editing without off-target effects.

Research grade: Predominantly supercoiled
Industrial Grade (100μg, 1 mg)
≥ 90% Supercoiled, ≤ 0.01 EU/μg endotoxin
HT Transfection Grade(10μg,30μg) > 90% Supercoiled, ≤ 0.1 EU/μg endotoxin 
How to store your vector? Please contact our customer service representatives or email support@genscript.com.