Custom CRISPR Plasmid Ordering

Search our CRISPR sgRNA Database to find pre-validated sgRNA sequences from the human and mouse genome.

Click here to order Broad Institute Plasmids that you can modify yourself.

Select custom designed CRISPR Plasmids below.

Plasmid Ordering Information: 1. Cas9 System
SpCas9
SpCas9 Nickase
SaCas9
SAM (Transcription Activation)

2. Delivery System
Plasmid
Lentiviral
AAV
ALL

3. Vector Type
All-in-one Vector
Dual/Multi Vector
ALL

4. Selection Markers
AmpR
PuroR
EGFP
NeoR
ZeoR
BeloR
ALL

5. Vector Selection
  Vector Cas9 System Delivery System Vector Type Selection Markers Cas9 Promoter Size Price
pSpCas9 BB-2A-GFP (PX458) SpCas9 Plasmid All-in-one Vector AmpR
EGFP
CBh 9.3 kb $199
pSpCas9 BB-2A-Puro (PX459) v2.0 SpCas9 Plasmid All-in-one Vector AmpR
PuroR
CBh 9.3 kb $199
pLentiCRISPR v2 SpCas9 Lentiviral All-in-one Vector AmpR
PuroR
EFS 9.3 kb $199
pLentiGuide-Puro SpCas9
SpCas9 Nickase
Lentiviral Dual Vector gRNA AmpR
PuroR
9.3 kb $199
pGS-gRNA SpCas9 Plasmid Dual Vector- gRNA AmpR 2.7 kb $199
pGS-gRNA-Neo SpCas9 Plasmid Dual Vector- gRNA AmpR
NeoR
4.9 kb $199
pAAV-SpGuide acceptor PX552 SpCas9 AAV Dual Vector- gRNA AmpR
EGFP
5.3 kb $199
pSpCas9n-BB (PX460) SpCas9 Nickase Plasmid All-in-one Vector AmpR CBh 8.5 kb $199
pSpCas9n-BB-2A-GFP (PX461) SpCas9 Nickase Plasmid All-in-one Vector AmpR
EGFP
CBh 9.3 kb $199
pSPCas9n-BB-2A-Puro (PX462) v2.0 SpCas9 Nickase Plasmid All-in-one Vector AmpR
PuroR
CBh 9.2 kb $199
pX601_AAV SaCas9 AAV All-in-one Vector AmpR CMV 7.4 kb $199
sgRNA (MS2) SAM Plasmid Multi Vector- gRNA AmpR 3.0 kb $199
lenti sgRNA(MS2)_zeo SAM Lentiviral Multi Vector- gRNA AmpR
ZeoR
BeloR
10.0 kb $199

6. Co-Vector Selection
  Vector Cas9 System Delivery System Vector Type Selection Markers Cas9 Promoter Size Price
pLentiCas9-Blast SpCas9 Lentiviral Dual Vector- Cas9 AmpR
BsdR
BleoR
EFS 12.8 kb Free with pLentiGuide-Puro
pLentiCas9-EGFP SpCas9 Lentiviral Dual Vector- Cas9 AmpR
EGFP
EFS 13.2 kb Free with pLentiGuide-Puro
pSpCas9 PX165 SpCas9 Plasmid Dual Vector- Cas9 AmpR CBh 8.2 kb Free with pSpCas9 PX165
pAAV-SpCas9 PX551 SpCas9 AAV Dual Vector- Cas9 AmpR pMecp2 7.4 kb Free with pAAV-SpCas9 PX551
pLentiCas9n-Blast SpCas9 Nickase Lentiviral Dual Vector- Cas9n AmpR
BsdR
BleoR
EFS 12.9 kb Free with pLentiGuide-Puro
lenti dCas9-VP64_Blast SAM Lentiviral Multi Vector- dCas9 AmpR
BlastR
BleoR
EF1a 14.1 kb $50 with pLenti_sgRNA(MS2)_zeo pSgRNA(MS2)
dCas9-VP_64_GFP SAM Lentiviral Multi Vector- dCas9 AmpR
EGFP
BleoR
EF1a 14.4 kb $50 with pLenti_sgRNA(MS2)_zeo pSgRNA(MS2)
lenti MS2-P65-HSF1_Hygro SAM Lentiviral Multi Vector- Activator AmpR
HygroR
BleoR
11.7 kb $50 with pLenti_sgRNA(MS2)_zeo pSgRNA(MS2)
MS2-P65-HSF_GFP SAM Lentiviral Multi Vector- Activator AmpR
EGFP
BleoR
11.4 kb $50 with pLenti_sgRNA(MS2)_zeo pSgRNA(MS2)

7. gRNA Sequence Selection
Synthesize my specified gRNA sequence
Design and synthesize gRNA sequences for my target sequence
*Plasmid name:
*Species:
*
*Plasmid name:
*Species:



8. Plasmid delivery
Research Grade 4μg Free
Transfection Grade 10μg +$29
Transfection Grade 30μg +$49
Transfection Grade 100μg +$99
Transfection Grade 1mg +$259

Service Requests

Need additional help? Share your experiment details with our PhD-level technical support staff. Click the “Submit to Technical Support” option below.

We recommend using 2 or more unique gRNA for each gene you wish to target, in order to increase the likelihood of successful genome editing without off-target effects.

Research grade: Predominantly supercoiled
Transfection Grade (100μg, 1 mg)
≥ 90% Supercoiled, ≤ 0.01 EU/μg endotoxin
HT Transfection Grade(10μg,30μg) > 90% Supercoiled, ≤ 0.1 EU/μg endotoxin 
How to store your vector? Please contact our customer service representatives or email support@genscript.com.