Custom CRISPR Plasmid Ordering

Search our CRISPR sgRNA Database to find pre-validated sgRNA sequences from the human and mouse genome.

Click here to order CRISPR plasmids with user-defined sgRNA sequences.

Select from the Broad Institute Plasmid Collection below.

Plasmid Ordering Information: 1. Cas9 System
SpCas9
SpCas9 Nickase
SaCas9

2. Delivery System
Plasmid
Lentiviral
AAV

3. Selection Markers
AmpR
PuroR
EGFP

4. Vector Selection
  Vector Cas9 System Delivery System Vector Type Selection Markers Cas9 Promoter Size Price
pSpCas9 BB-2A-GFP (PX458) Vector SpCas9 Plasmid All-in-one Vector AmpR
EGFP
CBh 9.3 kb $99
pSpCas9 BB-2A-Puro (PX459) Vector SpCas9 Plasmid All-in-one Vector AmpR
PuroR
CBh 9.2 kb $99
pLentiCRISPR v2 Vector SpCas9 Lentiviral All-in-one Vector AmpR
PuroR
EFS 14.9 kb $99
pSpCas9n-BB (PX460) Vector SpCas9 Nickase Plasmid All-in-one Vector AmpR CBh 8.5 kb $99
pSpCas9n-BB-2A-GFP (PX461) Vector SpCas9 Nickase Plasmid All-in-one Vector AmpR
EGFP
CBh 9.3 kb $99
pSpCas9n-BB-2A-Puro (PX462) V2.0 Vector SpCas9 Nickase Plasmid All-in-one Vector AmpR
PuroR
CBh 9.2 kb $99
pX601_AAV Vector SaCas9 AAV All-in-one Vector AmpR CMV 7.4 kb $99

5. Plasmid delivery
Research Grade 4μg Free
For increased quantities and/or transfection grade quality, please note in the service request box below.

Service Requests

Need additional help? Share your experiment details with our PhD-level technical support staff. Click the “Submit to Technical Support” option below.

We recommend using 2 or more unique gRNA for each gene you wish to target, in order to increase the likelihood of successful genome editing without off-target effects.

Research grade: Predominantly supercoiled
Transfection Grade (100μg, 1 mg)
≥ 90% Supercoiled, ≤ 0.01 EU/μg endotoxin
HT Transfection Grade(10μg,30μg) > 90% Supercoiled, ≤ 0.1 EU/μg endotoxin 
How to store your vector? Please contact our customer service representatives or email support@genscript.com.