Custom CRISPR Plasmid Ordering

Search our CRISPR sgRNA Database to find pre-validated sgRNA sequences from the human and mouse genome.

Click here to order CRISPR plasmids with user-defined sgRNA sequences.

Select from the Broad Institute Plasmid Collection below.

Plasmid Ordering Information:

1. Cas9 System:

SpCas9   SpCas9 Nickase   SaCas9  

2. Delivery System:

Plasmid   Lentiviral   AAV  

3. Selection Markers:

AmpR   PuroR   EGFP  

4. Vector Selection
  Vector Cas9 System Delivery System Vector Type Selection Markers Cas9 Promoter Size Price
pSpCas9 BB-2A-GFP (PX458) Vector SpCas9 Plasmid All-in-one Vector AmpR
EGFP
CBh 9.3 kb $99
pSpCas9 BB-2A-Puro (PX459) v2.0 Vector SpCas9 Plasmid All-in-one Vector AmpR
PuroR
CBh 9.2 kb $99
pLentiCRISPR v2 Vector SpCas9 Lentiviral All-in-one Vector AmpR
PuroR
EFS 14.9 kb $99
pSpCas9n-BB (PX460) Vector SpCas9 Nickase Plasmid All-in-one Vector AmpR CBh 8.5 kb $99
pSpCas9n-BB-2A-GFP (PX461) Vector SpCas9 Nickase Plasmid All-in-one Vector AmpR
EGFP
CBh 9.3 kb $99
pSpCas9n-BB-2A-Puro (PX462) V2.0 Vector SpCas9 Nickase Plasmid All-in-one Vector AmpR
PuroR
CBh 9.2 kb $99
pX601_AAV Vector SaCas9 AAV All-in-one Vector AmpR CMV 7.4 kb $99

5. Plasmid delivery
Research Grade 4μg Free
For increased quantities and/or Industrial Grade quality, please note in the service request box below.

Service Requests

Need additional help? Share your experiment details with our PhD-level technical support staff. Click the “Submit to Technical Support” option below.

We recommend using 2 or more unique gRNA for each gene you wish to target, in order to increase the likelihood of successful genome editing without off-target effects.

Research grade: Predominantly supercoiled
Industrial Grade (100μg, 1 mg)
≥ 90% Supercoiled, ≤ 0.01 EU/μg endotoxin
HT Transfection Grade(10μg,30μg) > 90% Supercoiled, ≤ 0.1 EU/μg endotoxin 
How to store your vector? Please contact our customer service representatives or email support@genscript.com.