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Step 1: Design your Gene 1. Sequence information send
* Fragment name (for your records only):
* Sequence:
Batch Orders (.xlsx)

2. Codon Optimization
Yes
No

3. Standard delivery:
400 ng GenParts DNA Fragment

Comments:

The optional Search Enzyme tool makes it easy to add restriction sites to flank your sequence.

Codon Optimization can improve protein expression levels and reduce gene sequence complexity. Optimization using GenScript's patented OptimumGene algorithm is a complimentary option for all gene synthesis orders. Codon optimization does not alter the amino acid sequence of protein products, but it introduces synonymous nucleotide substitutions within constraints you specify.  For more information, see our Codon Optimization Webinar.

Tips on completing this form:

1.Host expression organism:  You must specify the host in which you plan to express your codon-optimized gene.
2.Secondary host: (Optional) If you will be expressing the construct in multiple host species, you may choose to specify a secondary expression organism.
If you only plan to express your gene in single host, please don’t choose secondary host, as it will lower CAI for primary host.
3.Optimization start position and end position: if you want only a portion of your gene sequence to be subject to codon optimization, please specify the range to be optimized.
4.ORF start position and end position:  If your gene sequence contains non-coding regions before or after the ORF, please specify the ORF positions so that codon optimization is performed only within the ORF.
5.Restriction sites to avoid: if you want to ensure that codon optimization does not introduce specific restriction sites, please enter the restriction enzyme name and/or recognition sequence here.
6.Restriction sites to avoid: if you want to ensure that codon optimization does not remove specific restriction sites already present in the gene sequence, please enter the restriction enzyme name and/or recognition sequence here.
7.Stop codon needed: select "yes" or "no." If you want to specify the stop codon, enter it in the "Sequence" box.

If you have any questions about our optimization algorithm or how to customize it for your research needs, please contact gene@genscript.com or call 1-877-436-7274.

If you only plan to express your gene in single host, please don’t choose secondary host, as it will lower CAI for primary host.

Complex project? Not sure how to describe your needs on this form? Email gene@genscript.com or call 1-877-436-7274.

For most sequences, a quote will be instantly emailed to you stating our guaranteed turnaround time and price. For complex requests, our Ph.D.-level Technical Account Managers will email you a quote within one business day. 

 
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