- J Biotechnol. 2014-12; NULL NULL NULL
Process development of periplasmatically produced single chain fragment variable against epidermal growth factor receptor in Escherichia coli.
- Institute of Biochemical Engineering, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
|...Protein-L resin (GenScript USA Inc., Piscataway, NJ) was packed in Omnifit columns (6 mm x 20 mm, Sigma-Aldrich, St. Louis, MO) at pH7 (150 mM NaCl; 20 mM phosphate buffer). The scFv was be eluted upon pH shift to pH 2.5 (100 mM phosphate-citrate buffer)...|
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