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An unrecognized function for COPII components in recruiting the viral replication protein BMV 1a to the perinuclear ER

J Cell Sci.. 2016-10; 
Li J, Fuchs S, Zhang J, Wellford S, Schuldiner M, Wang X.
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Catalog Antibody ), anti-1a antiserum (Restrepo-Hartwig and Ahlquist, 1996), anti-His mAb (GenScript, Cat. no. A00186, Lot no. 12L000548) Get A Quote


Positive-strand RNA viruses invariably assemble their viral replication complexes (VRCs) by remodeling host intracellular membranes. How viral replication proteins are targeted to specific organelle membranes to initiate VRC assembly remains elusive. Brome mosaic virus (BMV), whose replication can be recapitulated in Saccharomyces cerevisiae, assembles its VRCs by invaginating the outer perinuclear endoplasmic reticulum (ER) membrane. Remarkably, BMV replication protein 1a (BMV 1a) is the only viral protein required for such membrane remodeling. We show that ER-vesicle protein of 14 kD (Erv14), a cargo receptor of coat protein complex II (COPII), interacts with BMV 1a. Moreover, the perinuclear ER localizatio... More


COPII; Erv14; Positive-strand RNA viruses; Protein perinuclear ER localization; Viral replication complexes; Viral replication protein targeting