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Mitogen-activated protein kinase (MAPK) dynamics determine cell fate in the yeast mating response.

J. Biol. Chem.. 2017-12; 
LiYang, RobertsJulie, AkhavanAghdamZohreh, Ha
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Biochemicals … 0.13MM). In all experiments, the microscope was programmed to acquire images for each fluorescence channel every 2 min. The pheromone stock used in this study was ordered from Genscript (catalog no. RP01002). We note … Get A Quote


In the yeast , the exposure to mating pheromone activates a prototypic mitogen-activated protein kinase (MAPK) cascade and triggers a dose-dependent differentiation response. Whereas a high pheromone dose induces growth arrest and formation of a shmoo-like morphology in yeast cells, lower pheromone doses elicit elongated cell growth. Previous population-level analysis has revealed that the MAPK Fus3 plays an important role in mediating this differentiation switch. To further investigate how Fus3 controls the fate decision process at the single-cell level, we developed a specific translocation-based reporter for monitoring Fus3 activity in individual live cells. Using this reporter, we observed strikingl... More


cell fate decision,cell signaling,differentiation,imaging,mitogen-activated protein kinase (MAPK),pheromone,single-cell analysis,yeast mating resp