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Catalog Antibody> | … 1:10,000 dilution, a gift from Dr. Paul Ahlquist at the University of Wisconsin-Madison), mouse anti- BMV 2apol (1:3,000 dilution), rabbit anti-HA (1:3,000 dilution, Thermo Fisher Scientific, cat #: 71-5500), mouse anti-His (1:3,000 dilution Genscript, cat #: A00186), mouse anti … | Get A Quote |
Viral infections universally rely on numerous hijacked host factors to be successful. It is therefore possible to control viral infections by manipulating host factors that are critical for viral replication. Given that host genes may play essential roles in certain cellular processes, any successful manipulations for virus control should cause no or mild effects on host fitness. We previously showed that a group of positive-strand RNA viruses enrich phosphatidylcholine (PC) at the sites of viral replication. Specifically, brome mosaic virus (BMV) replication protein 1a interacts with and recruits a PC synthesis enzyme phosphatidylethanolamine methyltransferase, Cho2p, to the viral replication sites tha... More