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An improved Tet-on system in microRNA overexpression and CRISPR/Cas9-mediated gene editing.

J Anim Sci Biotechnol. 2019-01; 
KangKang，HuangLian，LiQing，LiaoXiaoyun，DangQuanjin，YangYi，LuoJun，ZengYan，LiLi，GouDe
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Catalog Antibodies The following primary antibodies were used: PDCD4 (Santa Cruz Biotechnology, Santa Cruz, CA, USA, SC-13054, 1:1,000), NFAT5 (Santa Cruz Biotechnology, SC-13035, 1:200), FLAG (GenScript, Piscataway, NJ, USA, A00013, 1:1,000) Get A Quote

Abstract

Tetracycline (Tet)-regulated expression system has become a widely applied tool to control gene activity. This study aimed to improve the Tet-on system with superior regulatory characteristics.

Keywords

CRISPR/Cas9，Doxycycline，NFAT5，Tetracycline，micr
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