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Mdcop1 Ubiquitin E3 Ligases Interact With Mdmyb1 To Regulate Light-Induced Anthocyanin Biosynthesis And Red Fruit Coloration In Apple.

Plant Physiol.. 2012-10;  160(2):1011 - 1022
Yuan-Yuan Li, Ke Mao, Cheng Zhao, Xian-Yan Zhao, Hua-Lei Zhang, Huai-Rui Shu, and Yu-Jin Hao. State Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong
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Abstract

MdMYB1 is a crucial regulator of light-induced anthocyanin biosynthesis and fruit coloration in apple (Malus domestica). In this study, it was found that MdMYB1 protein accumulated in the light but degraded via a ubiquitin-dependent pathway in the dark. Subsequently, the MdCOP1-1 and MdCOP1-2 genes were isolated from apple fruit peel and were functionally characterized in the Arabidopsis (Arabidopsis thaliana) cop1-4 mutant. Yeast (Saccharomyces cerevisiae) two-hybrid, bimolecular fluorescence complementation, and coimmunoprecipitation assays showed that MdMYB1 interacts with the MdCOP1 proteins. Furthermore, in vitro and in vivo experiments indicated that MdCOP1s are necessary for the ubiquitination and degrad... More

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