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- PLoS ONE 2013;
Phosphorylation of mRNA decapping protein Dcp1a by the ERK signaling pathway during early differentiation of 3T3-L1 preadipocytes.
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Western blotting was conducted using the following specific antibodies: anti-Flag M2 (Sigma), anti-Myc (Genscript), anti-HA (Santa Cruz Biotech- nology), anti-GFP (Santa Cruz Biotechnology), anti-Dcp1a (Abcam), anti-Dcp2 (Abcam), anti-Edc3 (Abcam), anti-Edc4 (Abcam), and anti-Ddx6 (Abcam). | |
Abstract
Turnover of mRNA is a critical step in the regulation of gene expression， and an important step in mRNA decay is removal of the 5' cap. We previously demonstrated that the expression of some immediate early gene mRNAs is controlled by RNA stability during early differentiation of 3T3-L1 preadipocytes.
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