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Nup96 and HOS1 are Mutually Stabilized and Gate CONSTANS Protein Level, Conferring Long-day Photoperiodic Flowering Regulation in Arabidopsis

Plant Cell.. 2019; 
Cheng Z1, Zhang X2, Huang P1, Huang G3, Zhu J1, Chen F2, Miao Y4, Liu L5, Fu Y6, Wang X2.
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Proteins, Expression, Isolation and Analysis … the proteins were eluted with 2xSDS-PAGE loading buffer by heating at 95°C for 5 min, and<br> separated on 4-20% SurePAGEᵀᴹ precast gel (<b>Genscript</b>, M00657) followed by immunoblot<br> analyses … precast gel (8% SurePAGEᵀᴹ precast gel, M00663, <b>Genscript</b>, China) … Get A Quote


Lines of evidence indicate that nuclear pore complex profoundly affects the timing of plant flowering, but the underlying mechanisms remain poorly elucidated. Here, we report that Nucleoporin 96 (Nup96) acts as a negative regulator of long-day photoperiodic flowering in Arabidopsis. Through multiple approaches, we identified and verified an E3 ubiquitin ligase HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 1 (HOS1) interacting in vivo with Nup96. Nup96 and HOS1 proteins mainly localize and interact on nuclear membrane. Loss-of-function of Nup96 leads to destruction of HOS1 proteins without changing its mRNA abundance, which results in over-accumulation of the key activator of long-day photoperiodic flowering, C... More