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Multiple quality control mechanisms in the ER and TGN determine subcellular dynamics and salt-stress tolerance function of KORRIGAN 1

Plant Cell.. 2019; 
Nagashima Y1, Ma Z2, Liu X3, Qian X3, Zhang X4, von Schaewen A5, Koiwa H6.
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Catalog Antibody … processed in parallel, but separately. Immunoblotting was performed as described previously<br> using anti-GFP primary antibody (<b>Genscript</b>, catalog No. A01704, 1:4,000 dilution) and goat<br> anti-rabbit IgG HRP-conjugate (ThermoFisher, catalog No … Get A Quote


Among many glycoproteins within the plant secretory system, KORRIGAN1 (KOR1), a membrane-anchored endo-b1,4-glucanase involved in cellulose biosynthesis, provides a link between N-glycosylation, cell-wall biosynthesis, and abiotic stress tolerance. After insertion into the endoplasmic reticulum (ER), KOR1 cycles between the trans-Golgi network (TGN) and the plasma membrane (PM). From the TGN, the protein is targeted to growing cell plates during cell division. These processes are governed by multiple sequence motifs and also host genotypes. Here we investigated the interaction and hierarchy of known and newly identified sorting signals in KOR1, and how they affect KOR1 transport at various stages in the secreto... More