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A macromolecular α-glucan from fruiting bodies of Volvariella volvacea activating RAW264. 7 macrophages through MAPKs pathway.

Carbohydr Polym. 2020-02; 
Cui F1, Jiang L2, Qian L2, Sun W3, Tao T2, Zan X2, Yang Y4, Wu D5, Zhao X6.
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Cellular Analysis … cells, respectively. Additionally, all VGP fractions showed the negative LPS<br> contamination with LPS concentrations of less than 0.0001 EU/g by using <b>Genscript</b><br> ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit. Hence, it … Get A Quote

Abstract

A novel macromolecular polysaccharide VGPⅠ-a was purified from Volvariella volvacea fruiting bodies with ultrasound-assisted extraction, ion exchange and gel chromatography. VGPⅠ-a was proved as a α- glucan with Mw of 1435.6 kDa and contained a 1,4-linked d-Glcp backbone with the substitution at C-6 with 1-linked d-Glcp residue. Congo-red test, AFM and SEM analysis showed VGPⅠ-a had a triple-helical conformation and the interacted chains to form a small screw-rod and dispersed appearance. VGPⅠ-a had no cytotoxic effect on macrophage RAW264.7 cells in vitro and significantly enhanced the production and mRNA expression of NO, TNF-α, IL-6 and IL-1β in a dose-dependent manner. Further analyses demonstr... More

Keywords

Immunomodulatory activity; MAPKs pathways; Structural analysis; Volvariella volvacea; α-Glucan