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Biochemicals> | … Serum-free medium was replaced after cell adherence during culture for 24 h with medium containing various concentrations of TNF-alpha (0, 2 5, 5, 10 and 50 ng/mL; Genscript, USA) or DKK1 (0, 02 04 and 08 ng/mL; Genscript, USA) Six wells were used for each … | Get A Quote |
Rat dental follicle cells (rDFCs) function as precursor cells of periodontal tissues. Bone morphogenetic protein (BMP9) plays an important role in proliferation and differentiation. Tumour necrosis factor-alpha (TNF-alpha) is an important contributor to bone resorption. Wnt canonical pathway can be inhibited by Dickkopf 1 (DKK1). The aim of the study was to enhance the osteogenesis of BMP9 treated rDFCs in an inflammatory environment and elucidate the mechanism.,rDFCs were infected by adenoviruses expressing BMP9 (adBMP9). Expression levels of proteins and genes were measured by Western blotting and qPCR. The effect on osteogenesis was evaluated by measuring the activity of alkaline phosphatase (ALP), observati... More