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Capsid proteins of foot-and-mouth disease virus interact with TLR2 and CD14 to induce cytokine production

Immunology Letters. 2020-04; 
Yi-TeLin,Yen-PoChen,Chia-HsunFang,Pau-YiHuang,Shu-MeiLiang
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Abstract

The mechanism of recognition of the foot-and-mouth disease virus (FMDV) by host innate immune cells is not well-understood. In this study, we first found that binary ethylenimine inactivated-FMDV (BEI-FMDV) with structurally intact capsid activated TLR2, but not other TLRs, and this specific activation was blocked by anti-TLR2 Abs or knockout of TLR2. BEI-FMDV activated NF-κB to induce cytokines, notably interferon-β and IL-6, in a TLR2 and MyD88-dependent manner. Coexpression of TLR6 and CD14 showed additive effects on BEI-FMDV/TLR2-mediated activation of NF-κB. Further studies demonstrated that recombinant capsid proteins rVP1 and rVP3 of FMDV but not rVP0 bound directly with CD14 and TLR2. The rVP1- and r... More

Keywords

FMDVTLRCD14macrophagesIL-6