×
Resources » Reference Databases » Citations Database
Email to GenScript

Citations Search

  • PLoS Genet. 2013-06; 9 6 e1003559
  • Negative Regulation of the Novel norpAP24 Suppressor, diehard4, in the Endo-lysosomal Trafficking Underlies Photoreceptor Cell Degeneration.

  • J Lee, M Song, S Hong.
  • Subtropical Horticulture Research Institute, Jeju National University, Jeju, Korea

Products/Services Used Details Inquiry
...The polyclonal rabbit anti-arrestin2 antibody (GenScript, NY) is diluted 1:30,000. The anti-mouse or rabbit IgG HRP conjugated antibody was diluted 1:5000 in TBST containing 5% skin milk and the membrane was washed with TBST for 30 min...


PLoS Genet.

Abstract

Rhodopsin has been used as a prototype system to investigate G protein-coupled receptor (GPCR) internalization and endocytic sorting mechanisms. Failure of rhodopsin recycling upon light activation results in various degenerative retinal diseases. Accumulation of internalized rhodopsin in late endosomes and the impairment of its lysosomal degradation are associated with unregulated cell death that occurs in dystrophies. However, the molecular basis of rhodopsin accumulation remains elusive. We found that the novel norpAP24 suppressor, diehard4, is responsible for the inability of endo-lysosomal rhodopsin trafficking and retinal degeneration in Drosophila models of retinal dystrophies. We found that diehard4 encodes Osiris 21. Loss of its function suppresses retinal degeneration in norpAP24, rdgC306, and trp1, but not in rdgB2, suggesting a common cause of photoreceptor death. In addition, the loss of Osiris 21 function shifts the membrane balance between late endosomes and lysosomes as evidenced by smaller late endosomes and the proliferation of lysosomal compartments, thus facilitating the degradation of endocytosed rhodopsin. Our results demonstrate the existence of negative regulation in vesicular traffic between endosomes and lysosomes. We anticipate that the identification of additional components and an in-depth description of this specific molecular machinery will aid in therapeutic interventions of various retinal dystrophies and GPCR-related human diseases.

Keywords

Products or Services information in the article

...The polyclonal rabbit anti-arrestin2 antibody (GenScript, NY) is diluted 1:30,000. The anti-mouse or rabbit IgG HRP conjugated antibody was diluted 1:5000 in TBST containing 5% skin milk and the membrane was washed with TBST for 30 min...

Full Text FREE

Products or Services

Polyclonal Antibody Services|http://www.genscript.com/polyclonal_antibody_service.html?src=pullmenu

Most Popular Services

There are currently no Customer Testimonials.
Share your experience with us to scholarclub@genscript.com. Write a short (1 or 2 sentence) testimonial describing your experience with GenScript products or services. If your testimonial is selected for our website showcase, you'll receive 1,000 EzCoupon points.

 
*
*
*
*