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Two Pioneer Transcription Factors, Krüppel-Like Transcription Factor 4 and Glucocorticoid Receptor, Cooperatively Transactivate the Bovine Herpesvirus 1 ICP0 Early Promoter and Stimulate Productive Infection

J Virol. 2020; 
El-Mayet FS#, Sawant L#, Thunuguntla P, Zhao J, Jones C.
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Molecular Biology Reagents … made from the 5' terminus. Constructs with internal mutations (EP-943∆172-328, EP-943∆328- 369 638 and EP-943∆172-638) were synthesized by GenScript and inserted at pGL3-Basic Vector 370 (See Figure 4A for schematic of these constructs). 371 … Get A Quote

Abstract

An important site for bovine herpesvirus 1 (BoHV-1) latency is sensory neurons within trigeminal ganglia (TG). The synthetic corticosteroid dexamethasone consistently induces BoHV-1 reactivation from latency. Expression of four Krüppel-like transcription factors (KLF), i.e., KLF4, KLF6, PLZF (promyelocytic leukemia zinc finger), and KLF15, are induced in TG neurons early during dexamethasone-induced reactivation. The glucocorticoid receptor (GR) and KLF15 form a feed-forward transcription loop that cooperatively transactivates the BoHV-1 immediate early transcription unit 1 (IEtu1) promoter that drives bovine infected cell protein 0 (bICP0) and bICP4 expression. Since the bICP0 gene also contains a separate ea... More

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