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- Journal of Immunology Research 2020-06;
ILC2 Proliferated by IL-33 Stimulation Alleviates Acute Colitis in Rag1-/-Mouse through Promoting M2 Macrophage Polarization
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the expression of rIL-33 was induced by isopropyl-β-D-thiogalactoside and purified by using 6×His-Tagged Protein Purification Kit (Beijing CoWin Biotech, Beijing, China), followed by ToxinEraser Endotoxin Removal Kit (GenScript, Nanjing, China) to remove any endotoxin that might have come from the host cells. The purity of rIL-33 was more than 95% tested by SDSPAGE, and the endotoxin levels were less than 1 Eu/mg of protein using Toxin Sensor Chromogenic LAL Endotoxin Assay Kit (GenScript, Nanjing, China). | |
AbstractThis study was to identify functions of ILC2, a newly found innate lymphoid cell which mainly locates in mucosa organs like lungs and intestines, in IBD. We injected rIL-33 protein to C57/BL6 mouse to explore how IL-33 induces ILC2 proliferation. The results showed that ILC2 reached a proliferation peak at day 5 and expressed multiple surface markers like CD127, C-kit, CD69, CD44, ST2, CD27, DR3, MHCII, and CD90.2. ILC2 also expressed high quantity of IL-13 and IL-5 and few IL-17A which indicates a potentially immunological function in IBD development. Afterwards, we transferred sort purified ILC2 to Rag1-/- mouse given DSS to induce acute colitis in order to explore the innate function of ILC2. Data showed tha... More KeywordsMost Popular Services
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