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[Mutation of RAS gene in follicular-differentiated thyroid tumors and its significance]

Zhonghua Bing Li Xue Za Zhi. 2020-03-01; 
L P Liu, J Y Hao, H Pan, C Wang, P Yue
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PCR Cloning and Subcloning … Cloning and expression of mutant KRAS S65N and NRAS S65C. The full-length KRAS and NRAS cDNA clones were obtained from GenScript (Piscataway, NJ) and cloned into pcDNA3.1 under the control of the CMV promoter (Invitrogen, Carlsbad, CA) … Get A Quote


To investigate the frequency and clinical significance of RAS mutation in thyroid tumors with follicular differentiation. The samples and clinical data of 207 patients with thyroid follicular-differentiated tumors were collected at Shunyi Region Hospital of Beijing from January 2000 to December 2017, including 60 cases of follicular variant of papillary thyroid carcinoma (FVPTC), 42 cases of classical papillary thyroid carcinoma (CPTC), 26 cases of follicular thyroid carcinoma (FTC), 40 cases of follicular adenoma (FTA) and 39 cases of adenomatoid hyperplasia. BRAF V600E mutations were detected using immunohistochemical staining. FVPTC was divided into BRAF-like (BRAF V600E mutant) and RAS-like (without BRAF V... More


Immunohistochemistry, Polymerase chain reaction, Thyroid neoplasm