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- FEBS Open Bio 2020-12;
Long noncoding RNAs implicated in embryonic development in Ybx1 knockout zebrafish
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… The Ybx1 antibodies are from GenScript in Nanjing, China. Briefly, larvae were lysed with 100 µL 1x SDS sample buffer (62.5 mM Tris-HCl, 1% w/v SDS, 10% glycerol, 5% mercaptoethanol, pH=6.8), and the lysates were heated at 95 °C for 10 minutes … | |
AbstractY-box-binding protein 1 (Ybx1, YB-1), also known as Y-box transcription factor, is involved in a variety of biological processes and pathways, including embryogenesis, reproduction and development in vertebrates. Several non-coding RNAs regulate Ybx1 signaling. However, the role of long non-coding RNAs (lncRNAs) in embryogenesis remains incompletely understood. Here, we investigated the possible involvement of lncRNAs in Ybx1-mediated regulation of vertebrate development by performing systematic transcriptome analysis of RNA sequencing data derived from ybx1 homozygous mutant zebrafish on day5 (day5_ybx1 ) and wild type zebrafish on day5 and day6 (day5_ybx1 , day6_ybx1 ). We identified several lncRNAs affected ... More KeywordsDeep RNA Sequencing, Long Noncoding RNAs, Redox, YBX1, Zebrafish Embryo Development Most Popular Services
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