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- Front Vet Sci 2020-12;
Quantitative Proteomic Analysis of Duck Embryo Fibroblasts Infected With Novel Duck Reovirus
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… information and complete pathway classification results. WoLF PSORT (https://www.genscript. com/wolf-psort.html) is used to predict protein subcellular localization of eukaryotes. RNA Extraction and Quantitative Real-Time … | |
AbstractThe novel duck reovirus (NDRV) can cause hemorrhage and necrosis on the spleen of Pekin ducks; this disease has resulted in great economic losses to the duck industry. However, the molecular pathogenesis of NDRV remains poorly understood. In the current study, the quantitative proteomic analysis of NDRV-infected duck embryo fibroblasts was performed to explore the cellular protein changes in response to viral infection through iTRAQ coupled with the liquid chromatography (LC)-tandem mass spectrometry (MS/MS) method. A total of 6,137 proteins were obtained in cell samples at 24 h post-infection. Of these, 179 differentially expressed proteins (DEPs) were identified (cutoff set to 1.5-fold change), including 89 u... More Keywordscellular response, iTRAQ, interferon-stimulated genes, novel duck reovirus, proteomic analysis Most Popular Services |