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CRISPR/Cas9-mediated engineering of Escherichia coli for n-butanol production from xylose in defined medium

J Ind Microbiol Biotechnol. 2021-07; 
Ali Samy Abdelaal, Kamran Jawed, Syed Shams Yazdani
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Butanol production from agricultural residues is the most promising alternative for fossil fuels. To reach the economic viability of biobutanol production, both glucose and xylose should be utilized and converted into butanol. Here, we engineered a dual-operon-based synthetic pathway in the genome of E. coli MG1655 to produce n-butanol using CRISPR/Cas9 technology. Further deletion of competing pathway followed by fed-batch cultivation of the engineered strain in a bioreactor with glucose-containing complex medium yielded 5.4 g/L n-butanol along with pyruvate as major co-product, indicating a redox imbalance. To ferment xylose into butanol in redox-balanced manner, we selected SSK42, an ethanologenic E. coli st... More


Biofuels; Butanol; CRISPR/Cas9; Escherichia coli; Metabolic engineering; Xylose.