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Salmonella Typhimurium reprograms macrophage metabolism via T3SS effector SopE2 to promote intracellular replication and virulence

Nat Commun. 2021-02; 
Lingyan Jiang, Peisheng Wang, Xiaorui Song, Huan Zhang, Shuangshuang Ma, Jingting Wang, Wanwu Li, Runxia Lv, Xiaoqian Liu, Shuai Ma, Jiaqi Yan, Haiyan Zhou, Di Huang, Zhihui Cheng, Chen Yang, Lu Feng, Lei Wang
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Proteins, Expression, Isolation and Analysis … Protein samples were separated on a SurePAGE TM gel (GenScript #M00653) and were then transferred to polyvinylidene difluoride membranes using a wet transfer system. Membranes were blocked with tris-buffered saline with 0.1% (w/v) Tween 20 (TBST) containing 5% … Get A Quote


Salmonella Typhimurium establishes systemic infection by replicating in host macrophages. Here we show that macrophages infected with S. Typhimurium exhibit upregulated glycolysis and decreased serine synthesis, leading to accumulation of glycolytic intermediates. The effects on serine synthesis are mediated by bacterial protein SopE2, a type III secretion system (T3SS) effector encoded in pathogenicity island SPI-1. The changes in host metabolism promote intracellular replication of S. Typhimurium via two mechanisms: decreased glucose levels lead to upregulated bacterial uptake of 2- and 3-phosphoglycerate and phosphoenolpyruvate (carbon sources), while increased pyruvate and lactate levels induce upregulation... More