For each citation that was shared on social media (LinkedIn, Facebook, or Twitter) with the “@GenScript” tag, the author will be rewarded with a $10 Amazon gift card or 2,000 GS points.

The large nonstructural protein (NS1) of the human bocavirus 1 (HBoV1) directly interacts with Ku70, which plays an important role in virus replication in human airway epithelia

J Virol. 2021-12; 
Liting Shao, Kang Ning, Jianke Wang, Fang Cheng, Shengqi Wang, Jianming Qiu
Products/Services Used Details Operation
Proteins, Expression, Isolation and Analysis The supernatant was then collected, of which 80 μl was boiled in loading buffer as an input control, and the remaining supernatant was incubated with 100 μl of prewashed anti- Flag G1 affinity resin (#L00432, Genscript, Piscataway, NJ) followed by rotation at 4 °C overnight. Get A Quote


Human bocavirus 1 (HBoV1), an autonomous human parvovirus, causes acute respiratory tract infections in young children. HBoV1 infects well-differentiated (polarized) human airway epithelium cultured at an air-liquid interface (HAE-ALI). HBoV1 expresses a large nonstructural protein, NS1, that is essential for viral DNA replication. HBoV1 infection of polarized human airway epithelial cells induces a DNA damage response (DDR) that is critical to viral DNA replication involving DNA repair with error-free Y-family DNA polymerases. HBoV1 NS1 or the isoform NS1-70 per se induces a DDR. In this study, using the second-generation proximity-dependent biotin identification (BioID2) approach, we identified that Ku70 is a... More