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RBD conjugate vaccine with a built-in TLR1/2 agonist is highly immunogenic against SARS-CoV-2 and variants of concern

Chem Commun (Camb). 2022-01; 
Shi-Hao Zhou 1 , Ru-Yan Zhang, Hai-Wei Zhang, Yan-Ling Liu, Yu Wen, Jian Wang, Yu-Ting Li, Zi-Wei You, Xu-Guang Yin, Hong Qiu, Rui Gong, Guang-Fu Yang, Jun Guo
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Catalog Peptides IFN-γ precoated ELISpot kit (Dakewe, 2210005). In brief, the splenocytes were seeded at 1 × 106 cells per well and stimulated with Spike-derived overlapping peptides (GenScript, RP30020, 15 mers with 11 aa overlap) in vitro. After 18 h..., recombinant Spike RBD-His protein (GenScript, final concentration of 0.5 μg/mL) was added to the tubes in the presence or absence of the pooled antisera at 1:10 dilution for 1 h...Cells were washed with PBS and stained with His Tag Antibody iFluor 647 (GenScript, A01802) for 30 min. Get A Quote
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The coronavirus 2019 (COVID-19) pandemic is causing serious impacts in the world, and safe and effective vaccines and medicines are the best methods to combat the disease. The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein plays a key role in interacting with the angiotensin-converting enzyme 2 (ACE2) receptor, and is regarded as an important target of vaccines. Herein, we constructed the adjuvant-protein conjugate Pam3CSK4-RBD as a vaccine candidate, in which the N-terminal of the RBD was site-selectively oxidized by transamination and conjugated with the TLR1/2 agonist Pam3CSK4. This demonstrated that the conjugation of Pam3CSK4 significantly enhanced the anti-RBD antibody response and cellular... More