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LC3 subfamily in cardiolipin-mediated mitophagy: a comparison of the LC3A, LC3B and LC3C homologs

Autophagy. 2022-04; 
Marina N Iriondo , Asier Etxaniz , Yaiza R Varela , Uxue Ballesteros , Javier H Hervás , L Ruth Montes , Félix M Goñi , Alicia Alonso
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Externalization of the phospholipid cardiolipin (CL) to the outer mitochondrial membrane has been proposed to act as a mitophagy trigger. CL would act as a signal for binding the LC3 macroautophagy/autophagy proteins. As yet, the behavior of the LC3-subfamily members has not been directly compared in a detailed way. In the present contribution, an analysis of LC3A, LC3B and LC3C interaction with CL-containing model membranes, and of their ability to translocate to mitochondria, is described. Binding of LC3A to CL was stronger than that of LC3B; both proteins showed a similar ability to colocalize with mitochondria upon induction of CL externalization in SH-SY5Y cells. Besides, the double silencing of LC3A and L... More


Atg8; LC3/GABARAP-protein family; autophagosome; autophagy cargo recognition; lipid oxidation; lipid-protein interaction; membrane curvature; mitochondria; negatively charged phospholipids.