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Combining intramuscular and intranasal homologous prime-boost with a chimpanzee adenovirus-based COVID-19 vaccine elicits potent humoral and cellular immune responses in mice

Emerg Microbes Infect. 2022-12; 
Xingxing Li , Ling Wang , Jingjing Liu , Enyue Fang , Xiaohui Liu , Qinhua Peng , Zelun Zhang , Miao Li , Xinyu Liu , Xiaohong Wu , Danhua Zhao , Lihong Yang , Jia Li , Shouchun Cao , Yanqiu Huang , Leitai Shi , Hongshan Xu , Yunpeng Wang , Yue Suo , Guangzhi Yue , Jianhui Nie , Weijin Huang , Wenjuan Li , Yuhua Li
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Peptides and Peptide Library Freshly isolated lymphocytes (2.5 × 105 ) were transferred to the wells and stimulated at 37 °C for 24 h with a peptide pool (1 µg/mL per peptide, Genscript, Nanjing, China) derived from a peptide scan (15-mers with 11-residue overlaps) of the entire spike glycoprotein of SARS-CoV-2. Get A Quote

Abstract

The efficacy of many coronavirus disease 2019 (COVID-19) vaccines has been shown to decrease to varying extents against new severe acute respiratory syndrome coronavirus 2 variants, which are responsible for the continuing COVID-19 pandemic. Combining intramuscular and intranasal vaccination routes is a promising approach for achieving more potent immune responses. We evaluated the immunogenicity of prime-boost protocols with a chimpanzee adenovirus serotype 68 vector-based vaccine, ChAdTS-S, administered via both intranasal and intramuscular routes in BALB/c mice. Intramuscular priming followed by an intranasal booster elicited the highest levels of IgG, IgA, and pseudovirus neutralizing antibody titres among ... More

Keywords

Adenovirus-vectored vaccine; ChAdTS-S; SARS-CoV-2; intramuscular; intranasal.