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GAGE is a method for identification of plant species based on whole genome analysis and genome editing

Commun Biol. 2022-09; 
Lijun Hao , Wenjie Xu , Guihong Qi , Tianyi Xin , Zhichao Xu , Hetian Lei , Jingyuan Song
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Gene Synthesis The test of collateral cleavage activity was performed according to the procedures provided by Andrea Bonini et al.16, in which a 50 bp single-stranded DNA was synthesized (Fig. S1, Genscript Co. Ltd., China). Get A Quote


Whole genomes of plants should be ideal databases for their species identification, but unfortunately there was no such method before this exploration. Here we report a plant species identification method based on the whole Genome Analysis and Genome Editing (GAGE). GAGE searches for target sequences from the whole genome of the subject plant and specifically detects them by employing a CRISPR/Cas12a system. Similar to how Mendel chose Pisum sativum (pea), we selected Crocus sativus (saffron) to establish GAGE, in which we constructed a library containing all candidate target sequences. Taking a target sequence in the ITS2 region as an example, we confirmed the feasibility, specificity, and sensitivity of GAGE.... More