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Pold4 is dispensable for mouse development, DNA replication and DNA repair

Gene. 2023-01; 
Xueping Gu , Qinjin Dai , Peng Du , Ning Li , Jiahui Li , Simiao Zeng , Shuyi Peng , Shengjun Tang , Lei Wang , Zhongcheng Zhou
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Proteins, Expression, Isolation and Analysis 30 μg proteins of each sample were resolved by SurePAGETM precast gels (M00657, Genscript) and transferred to polyvinylidene difluoride membranes (ISEQ00010; Millipore). Get A Quote


The DNA polymerase delta (Pol δ), a heterotetramer of four subunits (Pol δ4), plays a pivotal role in DNA replication, as well as in DNA damage repair. Pold4, as the smallest subunit of Pol δ, is degraded in response to DNA damage or when entering into S-phase. This leads to the conversion of Pol δ4 to the trimeric complex Pol δ3. However, the contribution of Pold4 has not been fully elucidated in mammals. Cdm1, the Pold4 ortholog in Schizosaccharomyces pombe, is dispensable for cell growth and DNA damage repair, and there are no Pold4 orthologs in Saccharomyces cerevisiae. We previously generated a knockout mouse model of Pold3 and revealed its essential role in genome stability. Unexpectedly, we here fou... More


CRISPR; DNA damage; DNA repair; DNA replication; Pold4.